Preparation of allylinulin 131 I

Abstract

Inulin is used in physiological experiments, e. g. the study of the renal function and the measurement of the volume of extracellular water. As inulin is normally determined by colorimetric means, in which case other carbohydrates interfere, the introduction of a radioactivo tracer permits better measurements. In 1960 B r o o k s et al. [1] introduced an allyl group in the molecule of inulin and then added radioactive iodine to the double linkage thereof with a 48% yield in 24 hours. In 1964 Mit t a and C a m in [2] published a method giving a 70% yield in 1 hour by using radioactive iodine chloride. In 1967 Tubis et al. [3] prepared allylinulin and propargylinulin and marked it with 131 iodine, introducing certain modifications into the Brooks’ method. We have now prepared allylinulin with a 100% radiochemical yield by means of an easy technique, using a variation of method.